{"id":790,"date":"2017-09-25T12:09:32","date_gmt":"2017-09-25T06:39:32","guid":{"rendered":"https:\/\/babrone.avfu.ac.in\/blog\/?p=790"},"modified":"2018-11-12T10:07:19","modified_gmt":"2018-11-12T04:37:19","slug":"790","status":"publish","type":"post","link":"https:\/\/babrone.avfu.ac.in\/blog\/?p=790","title":{"rendered":"Fourth Issue"},"content":{"rendered":"<p style=\"text-align: justify;\"><strong>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span style=\"color: #000000;\"> We believe that behind every invention and discovery there is always a curious mind working. This section of BioNE, is dedicated to all those young curious minds who have been thinking and working on unexplored possibilities in the horizon of biological sciences. Today\u2019s learning process is no longer confined in books, rather it is based on questioning beyond the existing knowledge and searching for relevant answers. Here, we are inviting all those curious minds to put up questions and bring up a joint platform to share their ideas.&nbsp;Please mail&nbsp;your questions to&nbsp;<span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"mailto:bione@vetbifguwahati.ernet.in\">bione@vetbifguwahati.ernet.in<\/a><\/span><\/span><\/strong><\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li><span style=\"color: #000000;\"><span style=\"color: #ff0000;\"><strong>Q. &nbsp;How the graph of RT-PCR analysed?<\/strong><\/span><span style=\"color: #008080;\"><strong>&nbsp;-Priyanka Das, AIMT, B.Sc. (BT)<\/strong><\/span><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify;\"><span style=\"color: #000000;\"><span style=\"color: #008000;\"><strong>Answer:<\/strong><\/span> A real time PCR graph is a depiction of fluorescence intensity in every cycle of PCR reaction. Often, X-axis describes the n<sup>th<\/sup> number of cycle, whereas Y \u2013axis describes the level of florescence. Essentially, when a reaction is positive, the level of florescence increases with increase in number of cycles.<\/span><\/p>\n<p style=\"text-align: justify;\">&nbsp;<\/p>\n<ol start=\"2\">\n<li><span style=\"color: #000000;\"><strong><span style=\"color: #ff0000;\">Q. &nbsp;Why ultrapure water is used in HPLC?<\/span><span style=\"color: #008080;\"> \u2013Himadri Bhuyan, AIMT, B.Sc. (BT)<\/span><\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify;\"><span style=\"color: #000000;\"><span style=\"color: #008000;\"><strong>Answer:<\/strong><\/span> High Performance Liquid Chromatography (HPLC) is a highly sensitive detection and purification system for different molecules of synthetic and biological origin. It is very essential that we use the grade of water which is devoid of any ions so that it does not interfere with the purification process.<\/span><\/p>\n<p style=\"text-align: justify;\">&nbsp;<\/p>\n<ol start=\"3\">\n<li><span style=\"color: #000000;\"><strong><span style=\"color: #ff0000;\">Q. &nbsp;Is single nucleotide polymorphism (SNP) a mutational change?<\/span><\/strong>&nbsp; <strong><span style=\"color: #008080;\">-Raja Chakama, AIMT, B.Sc.(BT)<\/span><\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify;\"><span style=\"color: #000000;\"><span style=\"color: #008000;\"><strong>Answer:<\/strong><\/span> Mutation is a random process. An SNP is a mutation which is present in a constant position in a significant percentage in a population. SNPs play a significant role the process of evolution.<\/span><\/p>\n<p style=\"text-align: justify;\">&nbsp;<\/p>\n<ol start=\"4\">\n<li><span style=\"color: #000000;\"><strong><span style=\"color: #ff0000;\">Q. &nbsp;How will one pre-diagnose the presence of STD?<\/span> <span style=\"color: #008080;\">\u2013Minakshi Baruah, AIMT, B.Sc. (BT)<\/span><\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify;\"><span style=\"color: #000000;\"><strong><span style=\"color: #008000;\">Answer:<\/span><\/strong> Sexually transmitted diseases (STD) can be diagnosed in many ways, the most recent being by using molecular tools. In molecular diagnostic labs, probes are used which goes and binds with specific genes in the genome of the organism causing STD. Thus asymptomatic STDs or carriers can be diagnosed for STD.<\/span><\/p>\n<p style=\"text-align: justify;\">&nbsp;<\/p>\n<ol start=\"5\">\n<li><span style=\"color: #000000;\"><strong><span style=\"color: #ff0000;\">Q. &nbsp;Is it possible to encounter the control point of cancerous cells?&nbsp;<\/span><span style=\"color: #008080;\">-Minakshi Baruah, AIMT, B.Sc. (BT)<\/span><\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify;\"><span style=\"color: #000000;\"><strong><span style=\"color: #008000;\">Answer:<\/span><\/strong> Cancerous cells are nothing but the result of accumulation of mutations in the important genes of the cell. Due to random mutation process, cells lose their control over apoptosis and start dividing in an infinite manner. Till now, it has not become very clear as how much mutation is required for converting a normal cell to a cancerous one.<\/span><\/p>\n<p style=\"text-align: justify;\">&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; We believe that behind every invention and discovery there is always a curious mind working. This section of BioNE, is dedicated to all those young curious minds who have been thinking and working on unexplored possibilities in the horizon of biological sciences. Today\u2019s learning process is no longer confined&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[18,150],"tags":[53],"class_list":["post-790","post","type-post","status-publish","format-standard","hentry","category-qa","category-curiosityfourth-issue","tag-curiosity"],"_links":{"self":[{"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=\/wp\/v2\/posts\/790","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=790"}],"version-history":[{"count":3,"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=\/wp\/v2\/posts\/790\/revisions"}],"predecessor-version":[{"id":795,"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=\/wp\/v2\/posts\/790\/revisions\/795"}],"wp:attachment":[{"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=790"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=790"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/babrone.avfu.ac.in\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=790"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}